Antimicrobial property of recombinant Lactolisterin BU in vitro and its initial application in pork refrigerated storage

Abstract Lactolisterin BU is a novel bacteriocin identified from Lactococcus lactis in 2017. It exhibits antimicrobial activity against food spoilage and foodborne pathogens. In this study, was expressed Pichia pastoris ( P.pastoris ) isolated the supernatant of yeast culture for first time. found to exhibit broad spectrum rapid bactericidal bacterial pathogens, both gram-positive gram-negative ones, with minimum inhibition concentrations ranging within 10–60 ?g/mL. The recombinant rLactolisterin BU) also had an antioxidant effect resistant heating, acid–base, high-dose-saline treatments barely any hemolytic or cytotoxicity. Moreover, effectively suppressed growth pathogens; increases pH, total volatile basic nitrogen (TVB-N), thiobarbituric acid reactive substances (TBARS) pork samples; maintained high quality fresh during storage at 4 ?. Furthermore, inhibited three kinds bacteria pork-spoilage model. Taken together, could be promising preservative storage.